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KMID : 0370219810250040161
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Yakhak Hoeji
1981 Volume.25 No. 4 p.161 ~ p.165
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Drug-Biomacromolecule Interactions (II) Binding of Cephalothin and Cefazoline to Human Serum Albumin Using Difference Spectrophotometry
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김종국/Kim CK
양지선/안해영/김양배/우병설/Yang JS/Ahn HY/Kim YB/Yu BS
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Abstract
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The binding of two cephalosporins, cephalothin and cefazoline to human serum albumin (HSA) was studied by difference spectrophotometry using a spectrophotometric probe, 2-(4’’-hydroxy-benzeneazo) benzoic acid. The probe is strong visible absorbing material which interacts with serum albumin to give characteristic spectrophotometric peaks and provides the basis for a convenient assay to measure free and bound amounts in the presence of serum albumin and competitive drugs. The results obtained showed that the probe and cephalosporin compete for the same binding site on human serum albumin; thus the probe can be used to gauge the displacement of cephalosporins from human serum albumin. The data were interpreted on the basis of theory of multiple equilibria. The number of binding sites of human serum albumin for 2-(4’’-hydroxybenzeneazo) benzoic acid(HBAB), cephalothin and cefazoline appears to be 4. By using this technique the binding constants were found as follows: HSA-HBAB, 7.89 X 104M-1; HAS-cephalothin, 1.09 X 103M-1; HAS-cefazoline, 1.21 X 103M-1.
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